Phosphatidylinositol 4,5-Bisphosphate Induced Flunitrazepam Sensitive-GABAA Receptor Increase in Synaptosomes from Chick Forebrain |
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Authors: | Mariana Paula Cid Nancy Alicia Salvatierra Augusto Arce |
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Affiliation: | (1) Cátedra de Química Biológica, Departamento de Química, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba. Av, Vélez Sarsfield 1611, Córdoba, 5000, Argentina |
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Abstract: | The flunitrazepam sensitive-GABAA receptor density was increased by cytochalasins C and D at 37°C suggesting that microfilament depolymerization induces exposure to the radioligand of a GABAA receptor in synaptosomes (Pharm Biochem Behav 72 (2002) 497). Similarly, phosphatidylinositol-4,5-bisphosphate (1–5 μM), but not a mixture of phospholipids, induced an increase of GABAA receptors in synaptosomes. Furthermore, N-ethyl maleimide, an inactivator of the sensitive fusion protein, which interacts with GABAA receptor, abolished the receptor increase induced by phosphatidylinositol-4,5-bisphosphate. Together, the results suggest that phosphatidylinositol-4,5-bisphosphate, acts via microfilament depolymerization increasing the binding of the radioligand to receptors possibly by modulation of their interaction with proteins involved in trafficking and docking mechanisms. |
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Keywords: | Phosphatidylinositol-4,5-bisphosphate Microfilaments GABAA receptors Synaptosomes |
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