汞离子及铬离子对黄鳝脾免疫细胞的影响

采用毒性实验方法,用不同浓度的汞离子(Hg2+)、铬离子(Cr6+)分别处理黄鳝(Monopterusalbus),经1、2、4、8 d后,通过光镜观察黄鳝脾组织结构及免疫细胞数量的变化。结果表明,对照组黄鳝脾被膜较薄,未见明显的小梁,实质由红髓和白髓构成。白髓中淋巴细胞聚集成群,未见明显脾小结,但可见动脉周围淋巴鞘。红髓由脾索与脾窦组成。脾中有椭圆体,其末端向脾髓开放。黑色素巨噬细胞中心形成。经两种重金属离子分别染毒后的黄鳝脾与对照组相比,组织结构表现出相似的变化,即随着重金属离子浓度的增加和染毒时间的延长,脾组织中的黑色素巨噬细胞中心逐渐增大、增多,最后减少;黑色素巨噬细胞先增加后减少。淋巴组织逐渐松散,排列稀疏混乱,淋巴细胞界限逐渐不清晰,呈退化趋势,数量先增加后减少。粒细胞数量的变化趋势与淋巴细胞一致。红血细胞大量破坏,血窦扩张。

Effects of Hg2+ and Cr6+ on the Spleen Immune Cells of Monopterus albus

The changes of microscopic structures and the number of immune cells of spleen of the Ricefield Eel(Monopterus albus)were investigated after the fish were exposed separately to different concentrations of mercury ions(Hg2 +) and the Chromium ions(Cr6 +) for 1,2,4,and 8 days.The results showed that the splenic capsule was very thin and the trabeculae could not be found in the control group.The splenic parenchyma was composed of white pulp and red pulp.The lymphocytes of white pulp aggregated into groups.The ...