Force measurements of the disruption of the nascent polypeptide chain from the ribosome by optical tweezers |
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Authors: | Katranidis Alexandros Grange Wilfried Schlesinger Ramona Choli-Papadopoulou Theodora Brüggemann Dorothea Hegner Martin Büldt Georg |
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Affiliation: | Research Centre Jülich, Institute of Complex Systems (ICS-5), Jülich, Germany. a.katranidis@fz-juelich.de |
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Abstract: | We show that optical tweezers are a valuable tool to study the co-translational folding of a nascent polypeptide chain at the ribosome in real-time. The aim of this study was to demonstrate that a stable and intact population of ribosomes can be tethered to polystyrene beads and that specific hook-ups to the nascent polypeptide chain by dsDNA handles, immobilized on a second bead, can be detected. A rupture force of the nascent chain in the range of 10-50 pN was measured, which demonstrates that the system is anchored to the surface in a stable and specific way. This will allow in numerous future applications to follow protein folding using much lower forces. |
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