<Emphasis Type="Italic">In vitro</Emphasis> induction and identification of autotetraploids of <Emphasis Type="Italic">Dioscorea zingiberensis</Emphasis> |
| |
Authors: | Huang Heping Gao Shanlin Chen Lanlan Jiao Xiaoke |
| |
Institution: | (1) Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu, 210038, People’s Republic of China;(2) School of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, 230038, People’s Republic of China |
| |
Abstract: | Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics,
and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The
induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid
plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination
of morphological characteristics and for chemical assays. Six elite lines have been selected for further selection and breeding
into new varieties for commercial production. |
| |
Keywords: | Dioscorea zingiberensis Colchicine Medicinal plant Tetraploid |
本文献已被 SpringerLink 等数据库收录! |
|