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Polymorphic microsatellites in Buff-throated partridge developed by cross-species amplification
Authors:Xin Zhou  Yu Xu  Jianghong Ran  Bisong Yue  Lusha Cao  Jing Li
Institution:(1) Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, College of Life Science, Sichuan University, Wangjiang Road, Chengdu, Sichuan, 610064, China
Abstract:Microsatellite loci for the buff-throated partridge (Tetraophasis szechenyii), an endemic pheasant species of China, are here described for the first time. Twenty-five microsatellite markers from chicken and Japanese quail were tested on buff-throated partridge DNA by means of cross-amplification. Twenty (80%) primers yielded specific products and polymorphisms were tested in a wild population of buff-throated partridge. Twelve (48%) proved to be polymorphic with an average of two alleles per locus. Current results of buff-throated partridge microsatellites loci could be employed in population genetic studies and on other endangered pheasant species.
Keywords:Pheasant            Tetraophasis szechenyii
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