Glutathione peroxidase activity and release of glutathione from oxygen-deficient perfused rat heart. |
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Authors: | C Guarnieri F Flamigni C Rossoni-Caldarera |
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Affiliation: | Institute of Biochemistry, School of Medicine, University of Bologna, 40126 Bologna, Italy |
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Abstract: | The effect of cellular hypoxia on glutathione levels in rat hearts was determined. Hearts perfused with 95% N2–5% CO2 demonstrated a significant decrease in tissue reduced glutathione content when compared to control hearts perfused with 95% O2–5% CO2. The hypoxic perfusate contained reduced glutathione and its release was time dependent over a period of 60 minutes. The cellular depletion of oxidized glutathione and its release into coronary effluent were less evident with respect to reduced glutathione. Moreover during hypoxic perfusion we have observed a decrease of cytosol glutathione peroxidase activity. These results suggest that severe oxygen-deprivation causes in myocardial cells a significant perturbation of glutathione metabolism. |
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Keywords: | nitrous oxide tetrahydropteroylglutamic acid 5-methyltetrahydropteroylglutamic acid 10-formyltetrahydropteroylglutamic acid 5,10-methenyltetrahydropteroylglutamic acid |
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