A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers |
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| Authors: | A. Spada E. Caporali G. Marziani P. Portaluppi F. M. Restivo F. Tassi A. Falavigna |
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| Affiliation: | (1) CNR Centro Studi Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Univesità di Milano, Via Celoria 26, I-20133 Milano, Italy Fax: +039.02.26604330 E-mail: marziani@imiucca.csi.animi.it, IT;(2) Istituto di Genetica, Università di Parma, Via delle Scienze, I-43100 Parma, Italy, IT;(3) Istituto Sperimentale Orticultura, Via Paullese 28, I-20075 Montanaso Lombardo (MI), Italy, IT |
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| Abstract: | An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome 5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The closest marker to the sex determinant is the AFLP SV marker at 3.2 cM. Received: 26 March 1998 / Accepted: 30 April 1998 |
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| Keywords: | Asparagus Dioecious species Genetic map Molecular markers |
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