Structural information on nanomolecular systems revealed by FRET |
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| Authors: | Rolinski Olaf J Mathivanan Chinnaraj McNaught Gillian Birch David J S |
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| Institution: | Department of Physics, University of Strathclyde, John Anderson Building, 107 Rottenrow, Glasgow G4 0NG, UK. o.j.rolinski@strath.ac.uk |
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| Abstract: | Our newly developed fluorescence resonance energy transfer (FRET) based technique, fluorescence nanotomography (FN), is used to determine the morphology and dynamics of some soft materials and bio-molecules by attaching donor (D) fluorophores and acceptors (A) to the investigated structure and using fluorescence lifetime measurements to reveal the D-A distance distribution function rhoDA(r). We report the effect of the limited sizes of the donor and acceptor, effect of porous polymer, and molecular structure and phase transition in phospholipid bilayers. |
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