A microassay for the peptidase activity of enzymes utilizing ribonuclease S peptide as a substrate |
| |
| Authors: | S Levit M S Joshi |
| |
| Institution: | 1. Department of Biophysics The Weizmann Institute of Science Rehovoth, Israel;2. Department of Biodynamics The Weizmann Institute of Science Rehovoth, Israel |
| |
| Abstract: | A microassay for the peptidase activity of proteins obtained in minute amounts was devised. The method uses ribonuclease S peptide as a substrate. The substrate when cleaved is unable to reconstitute an active ribonuclease S complex. Therefore the loss in activity of the reconstituted complex is a measure of the peptidase activity. The method was previously tested with known peptidases such as clastase (9), chymotrypsin (8), and trypsin. In this work the peptidase activity of a protein related to a sperm-decapitating factor (1) is evidenced. |
| |
| Keywords: | To whom correspondence should be addressed |
| 本文献已被 ScienceDirect 等数据库收录！ |
