Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle |
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Authors: | Lingyan Zhang Min Yi |
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Institution: | (1) Forensic Identification Center, College of Criminal Investigation, Southwest University of Political Science and Law, 401120 Chongqing, China;(2) Chongqing Institutions of Higher Learning Center of Forensic Science Engineering and Research, Forensic Identification Center, Southwest University of Political Science and Law, 401120 Chongqing, China |
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Abstract: | The report is on an electrochemical biosensor with remarkably improved sensitivity toward nitrite. In this strategy, positively
charged gold nanoparticle (PCNA) is used in combination with multiwall carbon nanotubes (MWCNT) by electrostatic adsorption
for fabricating PCNA/MWCNT films. Then hemoglobin (Hb) biocatalyst will easily be attached to the surface of the combination
films aforementioned. After that, the Hb/PCNA films are immobilized onto the Hb/PCNA/MWCNT films through layer-by-layer assembly
technique. The (Hb/PCNA)2/MWNT/GC electrode thus prepared exhibits enhanced electrocatalytic behavior to the reduction of nitrite at −0.10 V versus
SCE in 0.05 M H2SO4 solution. On condition of the low detecting potential and low pH, interference caused by direct electrochemical oxidation or oxidizable
substances can be prevented. Therefore, the modified electrode shows fast response time, very high sensitivity, good selectivity
and stability. The current response of the sensor increases linearly with nitrite concentration from a range of 3.6 × 10−6 to 3.0 × 10−3 M with a detection limit(S /N = 3) of 9.6 × 10−7 M. |
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Keywords: | Positively charged gold nanoparticle Multiwall carbon nanotubes Electrocatalytic Hemoglobin Nitrite |
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