Spontaneous mutations changing the raffinose metabolism ofLactobacillus plantarum |
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Authors: | Siv Ahrné Göran Molin |
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Institution: | (1) Food and Environmental Microbiology, Dept. of Applied Microbiology, Chemical Center, P.O. Box 124, S-22100 Lund, Sweden |
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Abstract: | Lactobacillus plantarum ATCC 8014 grew poorly on raffinose agar plates, but large mutant colonies appeared in high frequency from a thin film of background growth. The -galactosidase and -galactosidase activities ofL. plantarum ATCC 8014 and a mutant strain were studied in static cultures and pH-controlled fermenter cultures. Both -galactosidase and -galactosidase production were inducible in the parental strain; the induction was not needed in the mutant. The -galactosidase activity of both strains was repressed by glucose but not by -methyl-D-glucoside. The mutant phenomenon might be an obstacle in connection to traditionalLactobacillus identification by means of carbohydrate fermentation. |
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Keywords: | -galactosidase" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-galactosidase Lactobacillus plantarum raffinose metabolism |
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