Quantitative micro-determination of lipid carbon in microorganisms |
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Authors: | O Holm-Hansen J Coombs B E Volcani P M Williams |
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Affiliation: | Institute of Marine Resources, University of California, La Jolla, California 92037 USA |
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Abstract: | A method is described for the quantitative determination of the carbon content of lipids extracted from microorganisms. The procedure depends upon conventional solvent extraction of the lipids followed by combustion of all lipid carbon to CO2. The CO2 is then measured by infrared gas analysis. The technique is extremely reliable and applicable for samples containing between 10–300 μg carbon. Results comparing the lipid content of various algae determined by this combustion technique show good agreement with the lipid content as determined by conventional gravimetric methods. |
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