Activation of phospholipase D by osmotic cell swelling |
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Authors: | Tomassen Sebastian F B van der Wijk Thea de Jonge Hugo R Tilly Ben C |
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Institution: | Department of Biochemistry, Erasmus University Medical Center, P.O. Box 1738, 3000DR Rotterdam, The Netherlands. |
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Abstract: | In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channels, but not the cell swelling-induced release of taurine, was largely inhibited in the presence of low concentrations of 1-butanol. In addition, hypotonicity-induced exocytosis, ATP release and subsequent endocytosis were found to be largely abrogated. The results support a model of cell volume regulation in which PLD plays an essential role in the cell swelling-induced vesicle cycling and in the activation of volume-sensitive anion channels. |
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