The isomaltulose synthesising enzyme ofSerratia
plymuthica |
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Authors: | M McAllister C T Kelly E Doyle W M Fogarty |
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Institution: | (1) Department of Industrial Microbiology, University College, Dublin 4, Ireland |
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Abstract: | Summary An intracellular enzyme was located inSerratia
plymuthica which produced isomaltulose from sucrose. The enzyme was purified giving a preparation with a specific activity of 1,285. It has pH and temperature optima of 6.0 and 30°C, respectively. The enzyme was stable retaining 100% activity after 2 weeks at 30°C. It had an isoelectric point at pH 9.0, a Mr of 79,500 and the Km for sucrose was 65.3mM. The enzyme converted 40% (w/v) sucrose to isomaltulose with an efficiency of 87%. |
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