Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS |
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Authors: | Günter Rudolph Karin Schilbach-Stückle Rupert Handgretinger Peter Kaiser Horst Hameister |
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Institution: | (1) Abteilung Klinische Genetik, Institut für Anthropologie und Humangenetik der Universität, Wilhelmstrasse 27, W-7400 Tübingen, Federal Republic of Germany;(2) Universitäts-Kinderklinik der Universität, Rümelinstrasse 19-23, W-7400 Tübingen, Federal Republic of Germany;(3) Abteilung Klinische Genetik der Universität, Frauenstrasse 29, W-7900 Ulm, Federal Republic of Germany |
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Abstract: | Summary A new human neuroblastoma cell line (LS) that originated from an abdominal tumor of a 16-month-old girl is presented; it was classified, according to Evans, as being stage III. Morphological (dense-core particles) and biochemical characteristics (dopamine- -hydroxylase, acetylcholinesterase, neuron-specific-enolase) confirmed the diagnosis. In addition to a slightly variable modal chromosome number of 48 or 49 (because of marker-chromosomes and autosomal trisomies), cytogenetic analysis revealed two constantly appearing chromosomes with homogeneously stained regions (HSR's). The karyo-type remained constant over 50 passages in vitro 49,XX, –12,+der5, + 17,+mar1,+mar2]. Double minutes were a rare phenomenon and appeared only in a few metaphases. In situ hybridization showed that some of the HSR's consisted of amplified N-myc copies. The distribution of the N-myc copies according to in situ hybridization signals along the HSR's was compared with the data of Southern and Northern blotting analyses. |
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