| 肠道病毒ECHO20血清型鉴定和VP1序列初步分析 |
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| 引用本文： | 马进,丁峥嵘,蒋立虹,刘建生,李亚雄,陈智豫,孟明耀,王萍,侯宗柳.肠道病毒ECHO20血清型鉴定和VP1序列初步分析[J].Virologica Sinica,2006,21(5):513-515. |
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| 作者姓名： | 马进 丁峥嵘 蒋立虹 刘建生 李亚雄 陈智豫 孟明耀 王萍 侯宗柳 |
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| 作者单位： | 中国医学科学院中国协和医科大学医学生物学研究所 云南昆明650118(马进，刘建生，侯宗柳)，云南省疾病预防控制中心 云南昆明650021(丁峥嵘，蒋立虹)，昆明市延安医院 云南昆明650051(李亚雄，陈智豫，孟明耀，王萍) |
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| 摘 要： | 肠道病毒(Enterovirus)是最常见的人类致病病毒之一,包括脊髓灰质炎病毒、柯萨奇A组病毒、柯萨奇B组病毒和ECHO病毒。一般认为大多数肠道病毒感染症状轻微或不明显,然而有时肠道病毒感染也可能是严重甚至致命的1]。2004年云南省潞西县局部地区发生小规模的甲肝流行,我们从当地
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| 关 键 词： | ECHO20病毒 逆转录聚合酶链反应 序列分析 |
| 收稿时间： | 2006-03-06 |
| 修稿时间： | 2006-07-06 |
VP1 Sequence Analysis and Serological Identification of a New Isolated Enterovirus ECHO20 |
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| MA Jin, DING Zheng-rong, JIANG Li-hong, LIUjian-shengl, LI Ya-xiong, CHEN Zhi-yu, MENG Ming-yao, WANG Ping, HOU Zong-liu.VP1 Sequence Analysis and Serological Identification of a New Isolated Enterovirus ECHO20[J].中国病毒学(英文版),2006,21(5):513-515. |
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| Authors: | MA Jin DING Zheng-rong JIANG Li-hong LIUjian-shengl LI Ya-xiong CHEN Zhi-yu MENG Ming-yao WANG Ping HOU Zong-liu |
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| Institution: | 1 .Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118 China;2.Yunnan Center for Disease Control and Prevention, Kunming 650021, China; 3. Yunnan Hospital, Kunming 650051, China |
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| Abstract: | Hepatitis A virus and ECHO20 viruses were isolated from faeces of hepatitis A patients in Luxi County, Yunnan Province. The combined and single serological neutralization antibody tests of enterovirus demonstrated that the patients were infected by both viruses. One strain, named LX0901, was selected for RT-PCR by using the general primers of VP1 of enterovirus. The product was purified and sequenced. The data were deposited in GenBank and compared with the complete VP1 sequence of 3 isolated ECHO20 strains. Analysis indicated that the LX0901 strain was ECHO20 virus, which was in agreement with serological neutralization tests. The variations of amino acids were not so high with an identity of more than 97%, but the variation were distributed throughout the genome. |
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| Keywords: | ECHO20 virus RT-PCR Sequence analysis |
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