Activation of the rat liver androgen-receptor complex |
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| Authors: | S Kyakumoto N Sato T Nemoto Y Ohara-Nemoto M Ota |
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| Institution: | Department of Biochemistry, Iwate Medical University, School of Dentistry, 19-1, Uchimaru, Morioka, Iwate-ken 020, Japan |
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| Abstract: | Activation of androgen receptor in rat liver cytosol was studied in vitro. The state of activation was judged by binding of 3H] R1881-receptor complex to chromatin. High ionic strength (0.4 M KCl as a final concentration) provoked the binding of 3H] R1881-receptor complex to chromatin at 0 degrees C. At low ionic strength, activation was very slow at 0 degrees C, but was very rapid at 25 degrees C and reached the maximum at 15 min of heating. |
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