The cytoskeleton of spreadingDictyostelium amoebae |
| |
Authors: | S Rubino J V Small |
| |
Institution: | (1) Present address: Department of Biology B-022, University of California San Diego, 92037 La Jolla, CA, USA;(2) Institute of Microbiology, University of Sasassari, Italy;(3) Institute of Molecular Biology, Austrian Academy of Science, Billrothstrasse 11, A-5020 Salzburg, Austria |
| |
Abstract: | Summary The cytoarchitectural elements ofDictyostelium discoideum amoeba have been visualized by light and electron microscopy in cells prepared with mixtures of glutaraldehyde and Triton-X-100. After negative staining, the peripheral regions of spreading amoebae show a complex meshwork of actin filaments, the majority of which were less than 0.25 microns in length. Multiple branch points, end to side abutments and cross-overs were characteristic features of the actin meshworks. Filopodia extending from the cell periphery consisted of bundles of actin filaments that penetrated into and merged with the actin meshworks in the spreading lamellae. Microtubules emanating from the nucleus associated body penetrated to differing extents into the actin meshworks, sometimes extending close to the cell periphery.Dictyostelium cytoskeletons preparted as described here should prove useful for further studies on the locomotory mechanism. |
| |
Keywords: | Actin Amoeba Cytoskeleton Dictyostelium |
本文献已被 SpringerLink 等数据库收录! |
|