Heterotypic trans-interaction of LI- and E-cadherin and their localization in plasmalemmal microdomains |
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Authors: | Baumgartner Werner Wendeler Markus W Weth Agnes Koob Rainer Drenckhahn Detlev Gessner Reinhard |
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Institution: | 1 Department of Cellular Neurobionics, Institute of Zoology, RWTH-Aachen University, D-52056 Aachen, Germany 2 Institute of Anatomy and Cell Biology, University of Würzburg, D-97070 Würzburg, Germany 3 Institute of Laboratory Medicine and Biochemistry, Virchow-Hospital of Charité Medical School Berlin, D-13353 Berlin, Germany 4 Laboratory for Molecular and Cellular Surgery, Department for Abdominal, Thoracic, Vascular and Transplant Surgery, University Hospital Leipzig, Liebigstr. 20, D-04103 Leipzig, Germany |
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Abstract: | Cadherins are calcium-dependent adhesion molecules important for tissue morphogenesis and integrity. LI-cadherin and E-cadherin are the two prominent cadherins in intestinal epithelial cells. Whereas LI-cadherin belongs to the subfamily of 7D (seven-domain)-cadherins defined by their seven extracellular cadherin repeats and short intracellular domain, E-cadherin is the prototype of classical cadherins with five extracellular domains and a highly conserved cytoplasmic part that interacts with catenins and thereby modulates the organization of the cytoskeleton. Here, we report a specific heterotypic trans-interaction of LI- with E-cadherin, two cadherins of distinct subfamilies. Using atomic force microscopy and laser tweezer experiments, the trans-interaction of LI- and E-cadherin was characterized on the single-molecule level and on the cellular level, respectively. This heterotypic interaction showed similar binding strength (20-52 pN at 200-4000 nm/s) and lifetime (0.8 s) as the respective homotypic interactions of LI- and E-cadherin. VE-cadherin, another classical cadherin, did not bind to LI-cadherin. In enterocytes, LI-cadherin and E-cadherin are located in different membrane regions. LI-cadherin is distributed along the basolateral membrane, whereas the majority of E-cadherin is concentrated in adherens junctions. This difference in membrane distribution was also reflected in Chinese hamster ovary cells stably expressing either LI- or E-cadherin. We found that LI-cadherin is localized almost exclusively in cholesterol-rich fractions, whereas E-cadherin is excluded from these membrane fractions. Given their different membrane localization in enterocytes, the heterotypic trans-interaction of LI- and E-cadherin might play a role during development of the intestinal epithelium when the cells do not yet have elaborate membrane specializations. |
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Keywords: | 7D-cadherin seven-domain cadherin AFM atomic force microscopy CHO Chinese hamster ovary CHOmLI CHO cells expressing LI-cadherin CHOmE CHO cells expressing E-cadherin BSA bovine serum albumin DMEM Dulbecco's modified Eagle's medium FCS fetal calf serum PEG polyethylene glycol RT room temperature HBSS Hanks' balanced salt solution PBS phosphate-buffered saline |
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