蛇床子素在大鼠肠缺血再灌注肺损伤模型中的肺保护机制研究

目的：探讨蛇床子素（Osthole）在大鼠肠缺血再灌注肺损伤（IIRI）中的保护机制。方法：健康雄性SD大鼠40只随机分为假手术组（sham组）、缺血再灌注组（I／R）和两个不同剂量给药组（Ost），其中缺血再灌注组和给药组采用夹闭大鼠肠系膜上动脉60min后松开动脉夹形成缺血再灌注模型。各给药组于缺血再灌注30min后分别给予5mg／kg、25mg／kg蛇床子素腹腔注射治疗。各实验组动物分别采用HE染色、肺组织水肿（W／D）比值、ELISA检测和免疫组化评价蛇床子素在缺血再灌注肺损伤后的肺保护机制。结果：缺血再灌注30min后，给药组大鼠HE，W／D、IL-1B和TNF—α结果均明显优于对照组（P〈0．05），并在25mg／kg剂量时表现出最大保护效果，同时Caspase-3蛋白表达水平也显著降低。结论：蛇床子素在大鼠肠缺血再灌注肺损伤后有一定的肺保护作用。

Lung Protection of Osthole Against Intestinal Ischemia-Reperfusion Lung Injury in Rats

Objective： To investigate the lung protection of osthole against intestinal ischemia-reperfusion lung injury in rats. Methods： A total of 40 SD rats were divided randomly into 4 groups： sham operated group （Sham）; I/R group （I/R）; Osthole 5 mg / kg group （Ost5） and osthole 25 mg / kg group （Ost25）. The animals in I/R group and osthole groups were exposed to the IIRI rat model. After 60 min of occlusion and 30min ofreperfusion, the rats in osthole groups were treated with 5 mg / kg, and 25 mg / kg osthole respectively. 30 min after the reperfusion, the pathological changes by hematoxylin and eosin （HE） dyeing were observed microscopically, wet, dry （W/D） ratio, IL-1β and TNF-α of lung tissues were examined for every groups. The expression of Caspase-3 protein in the lung tissue of every group was detected by immunohistochemistry. Osthole groups were better than that of I/R group （P〈0.05）. Results： Both the histopathologieal changes, W/D, IL-1β and TNF-α ofosthole groups were better than that of I/R group （P〈0.05）. Osthole could reduce the elevated level of caspase-3 protein induced by the ischemia and reperfusion. Conclusion： Osthole exerts protective effect against intestinal ischemia-reperfusion lung injury in rats.