| 一种白细胞介素-2缓释微球制备新方法及体外表征 |
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| 引用本文： | 陈顺,赵海平,徐健雄,陈音,郭秋圆,黄袭雯,陶安琪,李晓鸣,张向荣,林清.一种白细胞介素-2缓释微球制备新方法及体外表征[J].现代生物医学进展,2013(33):6456-6459,6420. |
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| 作者姓名： | 陈顺 赵海平 徐健雄 陈音 郭秋圆 黄袭雯 陶安琪 李晓鸣 张向荣 林清 |
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| 作者单位： | [1]上海市第十人民医院,上海200072 [2]上海交通大学药学院,上海200240 [3]上海交通大学医学院附属新华医院,上海200092 [4]深圳赛宝儿生物药业有限公司,广东深圳518129 |
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| 基金项目： | 国家自然科学基金项目（81001416）;医工交叉项目（YG2010ms11） |
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| 摘 要： | 目的：开发一种白细胞介素-2（m-2）长效缓释微球剂型。方法：采用S／O／W法制备了白介素-2因子多糖微粒的PLGA微球，考察了微球的表面形态、粒径分布等，并且运用ELISA方法考察了微球的体外释放效果。结果：本方法制备的白介素-2因子微球光滑圆整，粒径分布较均匀，体外缓释达32天，累积释放率近90％。结论：本方法制备的白介素-2因子微球，不仅具有有效地保护IL-2蛋白活性，同时实现长效缓释的目标，是一种可行的蛋白缓释方案。
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| 关 键 词： | 白介素-2 缓释微球 肿瘤 |
A New Method for Interleukin-2 Release Microsphere Preparation and in Vitro Characterization |
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| CHEN Shun,ZHAO Hai-ping,XU Jian-xiong,CHEN Yin,GUO Qiu-yuan,HUANG Xi-wen,TAO An-qi,LI Xiao-min,ZHANG Xiang-rong,LIN Qing.A New Method for Interleukin-2 Release Microsphere Preparation and in Vitro Characterization[J].Progress in Modern Biomedicine,2013(33):6456-6459,6420. |
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| Authors: | CHEN Shun ZHAO Hai-ping XU Jian-xiong CHEN Yin GUO Qiu-yuan HUANG Xi-wen TAO An-qi LI Xiao-min ZHANG Xiang-rong LIN Qing |
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| Institution: | 1 Shanghai Tenth People＇s Hospital, Shanghai, 200072, China; 2 Shanghai JiaoTong University School ofPharnacy, Shanghai, 200240, China; 3 Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; 4 Shenzhen SCIPROGENBio-pharmaceutical Co., LM Guangdong, Shenzhen, 518129, China) |
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| Abstract: | Objective： To develop an interleukin-2 （IL-2） long-term sustained release microsphere formulations. Methods： dextran glassy particles were prepared by a unique method of low temperature aqueous-aqueous ＂emulsion＂, and then the IL-2-1oaded dextmn glassy particles were encapsulated into PLGA microspheres by the method of solid-in-oil-in-water （S/OB/~） emulsion-evaporation technique. The microspheres were characterized by SEM and size distribution. In vitro release profiles of IL-2 loaded microspheres were plotted by Human IL-2 Quantikine ELISA Kit. Result： The IL-2-1oaded microspheres possessed spherical shape and smooth surface. The size distribution demonstrated that average spherical diameter of IL-2 microspheres was equal to 29.427 μm. The cumulative release in vitro was about 90% after 32 days. Conclusion： This method effectively protected the bioactivity of IL-2F and microspheres exhibited an ideal sustained release behavior. |
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| Keywords: | Interleukin-2 Microspheres Tumor |
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