MiR-26a在人垂体腺瘤组织及血清中表达的研究

目的：miRNA遍及生命体的发生、发育、分化和死亡的过程。它在肿瘤、心血管、糖尿病等多种疾病的各个阶段中起到调控癌基因作用。miRNA在垂体腺瘤中异常表达，且影响垂体腺瘤的增殖、侵袭及凋亡情况。本研究通过探讨miRNA家族中的miR-26a在垂体腺瘤组织及血清中的表达变化情况，为垂体瘤的早期诊断及疗效监测提供依据，以便更好的指导临床诊断及治疗工作。方法：收集哈尔滨医科大学附属第四医院微创神经外科手术切除并经病理证实的垂体腺瘤20例，取其组织及采集血清标本：年龄在20-74岁（平均50岁），术前均未进行任何治疗。既往无内分泌疾病的正常死亡人的垂体组织及其血清标本20例作为对照组。采用实时定量聚合酶链式反应（Real-timePCR）方法分别检测垂体腺瘤病人和正常人组织及血清中的miRNA-26a的表达情况。用SPSSl3．0统计分析软件运用Mann—WhitneyU检验方法对数据进行统计学分析。结果：miRNA-26a在垂体腺瘤组织中的表达量为22．30，正常垂体组织中的表达量为23．38，垂体腺瘤患者血清中miRNA-26a的表达表达量为25．04，正常对照组血清中的表达量为24．95，垂体腺瘤组织中的表达较正常垂体组织中的表达明显升高（P〈0．05），垂体腺瘤患者血清与正常人血清中miRNA-26a的表达无明显差异（P〉0．05）。结论：垂体腺瘤组织中miRNA-26a的高表达与血清学检测miRNA-26a的正常表达，为预防脑垂体腺瘤的发生和发展提供了重要的临床诊断依据。

The Exploration of MiR-26a Expression in Serum and Tumor Tissues of Patients with Pituitary Adenoma

Objective： MiRNA spread all the life body occurrence, development, differentiation and death process. It serves to reg- ulate oncogene role in every stage of tumor, cardiovascular, diabetes and other diseases. The abnormal expression of miRNA in pituitary adenoma, and the influence of pituitary adenoma proliferation, invasion and apoptosis. In this study, the expression changes ofmiRNA in the family miR-26a in pituitary adenoma tissue and serum, and provide evidence for early diagnosis and therapeutic monitoring of pitui- tary tumor, in order to better guide the clinical diagnosis and treatment. Methods： Totally, 20 consecutive patients with pituitary adenoma were recruited. The manager was 50.1 years with an age range of 20-74 years. All patients were operated in the department of minimal invasive neurosurgery of our hospital, and were confirmed as pituitary adenoma via postoperative pathology. Tumor tissues and serum were collected and analyzed with Real-time PCR. We use SPSS13.0 software package for statistical analysis and Mann-Whitney U test was used if necessary. Results： Expression of miRNA-26a in pituitary adenomas is 22.30, expression of normal pituitary tissues for 23.38, the expression of serum miRNA-26a in patients with pituitary adenomas, the expression amount of 25.04, the normal control group, the expression in serum for 24.95. Conclusion： Increased miR-26a expression was detected in pituitary adenoma tissues（P〈0.05 ）, whereas in blood samples no significance was found between the two groups （P〉0.05）. The normal expression and serological detection of miRNA-26a miRNA-26a in pituitary adenoma tissues, which provides the important basis for clinical diagnosis to prevent the occur- fence and devel- opment of pituitary adenoma.