Quantitative and qualitative transitions of methanogen community structure during the batch anaerobic digestion of cheese-processing wastewater |
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Authors: | Changsoo Lee Jaai Kim Seung Gu Shin Vincent O’Flaherty Seokhwan Hwang |
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Institution: | (1) Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore;(2) Microbial Ecology Laboratory, Department of Microbiology and Environmental Change Institute (ECI), National University of Ireland, Galway, Ireland;(3) School of Environmental Science and Engineering, POSTECH, San 31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk, 790-784, South Korea; |
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Abstract: | Qualitative and quantitative shifts in methanogen community structure, associated with process performance data, were investigated
during the batch anaerobic digestion of a cheese-processing wastewater, whey permeate. Denaturing gradient gel electrophoresis
(DGGE) and real-time PCR techniques were applied to obtain qualitative and quantitative microbial data sets, respectively,
based on methanogen 16S rRNA genes. Throughout the operation, dynamic variations in both qualitative and quantitative community
structures were observed, with repeated shifts in dominance between the aceticlastic Methanosarcinaceae (suggested mainly by the detection of a Methanosarcina-like population) and the hydrogenotrophic Methanomicrobiales (suggested mainly by the detection of a Methanofollis-like population). This trend corresponded well to the diauxic utilization of acetate and longer-chain fatty acids (C3–C6), mainly propionate. Joint-plot non-metric multidimensional scaling (NMS) analysis demonstrated that the qualitative and
quantitative community shifts had significant correlations with the composition of residual organic acids and the methane
production rate, respectively. This suggests the potential use of microbial community shift analysis as an indicative tool
for diagnosing anaerobic digestion processes. The results suggest that more attention should be directed to quantitative,
as well as qualitative, approaches for a better understanding of anaerobic digestion, particularly in terms of biogas production
efficiency, under dynamic and transitional conditions. |
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