In vivo bioconversion of tetrahydroisoquinoline by recombinant coclaurine N-methyltransferase |
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Authors: | Morishige Takashi Choi Kum-Boo Sato Fumihiko |
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Institution: | Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Japan. |
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Abstract: | Coclaurine N-methyltransferase from Coptis japonica catalyzes the N-methylation of coclaurine as well as simple tetrahydroisoquinoline. We examined the possibility of converting 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline into its N-methylated product using transgenic Escherichia coli, which expressed recombinant coclaurine N-methyltransferase, without the addition of a methyl-group donor. Transgenic E. coli successfully N-methylated the substrate added to the medium and excreted the product. Limitation of bioconversion by the supply of methyl-group donor is discussed. |
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