In vitro organogenesis and plant formation in cucumber |
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Authors: | N Selvaraj A Vasudevan M Manickavasagam A Ganapathi |
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Institution: | (1) Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India;(2) Department of Botany, Periyar E.V.R. College (Autonomous), Tiruchirappalli, Tamil Nadu, 620023, India |
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Abstract: | In vitro organogenesis was achieved from callus derived from hypocotyl explants of Cucumis sativus L. cv. Poinsett 76. Calli were induced from hypocotyl explants excised from 7-d-old seedlings grown on Murashige and Skoog
(MS) medium containing 87.64 μM sucrose, 0.8 % agar, 3.62 μM 2,4-dichlorophenoxy acetic acid and 2.22 μM 6-benzyladenine (BA).
Regeneration of adventitious buds from callus (25 shoots explant−1) was achieved on MS medium supplemented with 8.88 μM BA, 2.5 μM zeatin and 10 % coconut water after two subcultures in the
same medium at 30-d interval. Gibberellic acid (1.75 μM) favoured shoot elongation and indole 3-butyric acid (7.36 μM) induced
rooting. Rooted plants were hardened and successfully established in soil. |
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Keywords: | adventitious bud callus Cucumis sativus L hypocotyl |
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