Genetic analysis of anther culture response in wheat carrying alien translocations

A bread wheat cultivar, Saratovskaya 29, (S29), its nearly isogenic lines carrying alien translocations [Lr9 from Aegilops umbellulata (Eg29) and (Lr19) from Agropyron elongatum (Ps29)] and two F₁ hybrids between three nearly isogenic lines of S29 that differed by the Lr19+Rht1,Pro1+Pro2 and Ppd1+Ppd2 gene complexes, namely the S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) F₁ and the S29 (Pro1+Pro2)/S29 (Lr19+Rht1) F₁ were studied for their culture response with the following results. (1) Translocations with Lr9 and Lr19 decreased embryo frequency and green plant regeneration. (2) Both F₁ hybrids showed a decrease in embryo frequency. One of the F₁ hybrids, S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) showed a decrease, with respect to S29 for green plant regeneration; the other F₁ S29 (Pro1+Pro2)/S29 (Lr19+Rht1), equalled S29 for green plant regeneration. (3) The gene complex of the F₁ hybrid S29 (Pro1+Pro2)/S29 (Lr19+Rht1) was better than that of the F₁ hybrid S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) for embryo induction and green plant regeneration. This effect was possibly induced by interactions between the Pro1+Pro2 and Lr19+Rht1 genes or was the result of direct actions of the Pro1+Pro2 genes.