Stability Study of Crude Dextransucrase from <Emphasis Type="Italic">Leuconostoc citreum</Emphasis> NRRL B-742 |
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Authors: | Maria Cristiane Rabelo Claudia M L Fontes Sueli Rodrigues |
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Institution: | (1) Departamento de Tecnologia de Alimentos, Universidade Federal do Ceara, Av. Mister Hull, 2977, Bloco 858 – Campus do Pici, Fortaleza, CE, 60356-000, Brazil; |
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Abstract: | In the present work, the stability of crude dextransucrase from Leuconostoc citreum B-742 was evaluated in synthetic and in cashew apple juice culture broth. Optimum stability conditions for dextransucrase
from L. citreum B-742 were different from the reported for its parental industrial strain enzyme (L. mesenteroides B-512F). Crude dextransucrase, from L. citreum B-742, produced using cashew apple juice as substrate, presented higher stability than the crude enzyme produced using synthetic
culture medium, showing the same behavior previously reported for dextransucrase from L. mesenteroides B-512F. The crude enzyme presented good stability in cashew apple juice for 48 h at 25°C and pH 6.5. |
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Keywords: | Agriculture substrates Enzyme stability Glycosyltransferase Fermentation Leuconostoc citreum |
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