Determination of low levels of poly(ethylene glycol) 400 in plasma and urine by capillary gas chromatography-selected ion-monitoring mass spectrometry after solid-phase extraction |
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Authors: | Christina Fakt Magnar Ervik |
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Affiliation: | Bioanalytical Chemistry, Astra Hässle AB, S-431 83, Mölndal, Sweden |
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Abstract: | A convenient and sensitive method for the quantitative determination of poly(ethylene glycol) 400 in plasma and urine with capillary gas chromatography-mass spectrometry has been developed. The sample preparation involves solid-phase extraction with subsequent derivatization with heptafluorobutyric anhydride, which proved to give the most stable derivative. The derivatization procedure was optimized using experimental design, and different solid-phase extraction columns were evaluated. The limit of quantitation was 1 μmol/l (0.4 μg/ml) for both plasma and urine. |
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Keywords: | Poly(ethylene glycol) 400 |
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