Fermentation ofCandida utilis for uricase production |
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Authors: | Vladimír Adámek Martina Suchová Kate?ina Demnerová Blanka Králová Ivan Fo?t Petr Morava |
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Institution: | (1) Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague;(2) Department of Chemical and Food Process Equipment Design, Czech Technical University, Prague, Czechoslovakia |
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Abstract: | Summary Conditions for the production of microbial uricase byCandida utilis were studied. For the selected strain, hypoxanthine proved to be the most effective inducer of uricase formation. The highest values of biomass as well as uricase activity in the mechanically agitated fermentor were obtained under the following conditions: 50 h, rotation impeller speed 7 s–1, air flow rate 1.25×10–5 m3s–1, concentration of inducer 0.1%.List of symbols
b
width of baffle, m
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c
length of baffle, m
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D
diameter of cylindrical fermentor, m
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d
diameter of impeller, m
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d
1
diameter of impeller disc, m
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Fr
m
impeller Froud number
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g
gravitional acceleration, ms–2
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H
height of batch surface above bottom, m
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H
2
height of impeller disc above bottom, m
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h
height of impeller blade, m
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Kp
g
flow rate number
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L
length of impeller blade, m
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N
rotational speed of impeller, s–1
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Re
m
impeller Reynolds number
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T
time, h
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V
volume of batch, m3
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V
g
air (gas) flow rate, m3s–1
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x
mass fraction of the dry matter of cells
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x
0
initial value of the mass fraction of the dry matter of cells
- r
volume fraction of the dry matter of cells
- <eta<1
viscosity of pure liquid, Pa s
-
viscosity of batch (suspension of microbial suspension), Pa s
-
density of batch, kg m–3 |
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Keywords: | Uricase production Candida utilis Inducer of uricase production Fermentation kinetics |
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