Hemolysis of rabbit erythrocytes in the presence of copper ions |
| |
Authors: | James M Caffrey Harry A Smith John C Schmitz Andrea Merchant Earl Frieden |
| |
Institution: | Department of Chemistry, Florida State University, Tallahassee 32306-3006. |
| |
Abstract: | The hemolysis of red blood cells (RBC) induced by Cu(II) is modified by ceruloplasmin (Cp) and albumin. The time course of hemolysis for rabbit RBC by Cu(II) consisted of two parts, an induction period followed by a catastrophic lysis period. The induction period decreased and the lysis rate increased with increasing Cu(II) concentration. Cp or albumin, modified Cu(II) induced hemolysis, by increasing the duration of the induction period and decreasing the overall rate of hemolysis of RBC. The catastrophic lysis period coincided with a sharp increase in the formation of metHb within the cell and in a rapid uptake of Cu(II). The presence of Cp led to an increase in the induction period prior to the rapid increase in metHb formation and in Cu(II) uptake. Porcine Cp was prepared with either two or three nonprosthetic copper binding sites (sites where Cu(II) is easily removed by passing over Chelex-100). Cp with three nonprosthetic binding sites gave more protection than Cp with two. Likewise, albumin can be prepared with three and five nonprosthetic copper binding sites. The albumin with five sites gave more protection than the albumin with three sites. |
| |
Keywords: | Index Entries" target="_blank">Index Entries Red blood cells ceruloplasmin albumin Cu(II) uptake MetHb formation Cu(II) induced hemolysis ceruloplasmin-Cu(II) complexes hemolysis inhibition by ceruloplasmin |
本文献已被 SpringerLink 等数据库收录! |