Exopectinases produced by Aspergillus niger in solid-state and submerged fermentation: a comparative study |
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Authors: | Díaz-Godínez G Soriano-Santos J Augur C Viniegra-González G |
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Institution: | Area de Biotecnología del Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Ixtacuixtla Tlaxala, México. |
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Abstract: | Exopectinase production by Aspergillus niger was compared in submerged fermentation (SmF) and solid-state fermentation (SSF). SSF was carried out using polyurethane foam
(PUF) as the solid support. The purpose was to study the effect of sucrose addition (0 or 40 g/l) and water activity level
(A
w=0.99 or 0.96) on the level of enzyme activity induced by 15 g/l of pectin. Mycelial growth, as well as extracellular protease
production, was also monitored. Sucrose addition in SmF resulted in catabolite repression of exopectinase activity. However,
in SSF, an enhancement of enzyme activity was observed. Protease levels were minimal in SSF experiments with sucrose and maximal
in SmF without sucrose. Exopectinase yields (IU/g X) were negligible in SmF with sucrose. The high levels of exopectinase with sucrose and high A
w in SSF can be explained by a much higher level of biomass production without catabolite repression and with lower protease
contamination. Journal of Industrial Microbiology & Biotechnology (2001) 26, 271–275.
Received 05 July 2000/ Accepted in revised form 27 January 2001 |
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Keywords: | : exopectinase solid-state fermentation polyurethane enzyme yield |
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