Molecular analysis of plasmid pAP4 from Acetobacter pasteurianus |
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| Authors: | Jozef Grones Miroslava Kretová |
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| Institution: | (1) Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-2, SK-84215 Bratislava 4, Slovakia;(2) Food Research Institute, Priemyselná 4, P.O. Box 25, SK-82475 Bratislava 26, Slovakia |
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| Abstract: | The complete nucleotide sequence of plasmid pAP4 isolated from Acetobacter pasteurianus 2374T has been determined. Plasmid pAP4 was analysed and found to be 3,870 bp in size with a G+C content of 50.1%. Computer assisted
analysis of sequence data revealed 2 possible ORFs with typical promoter regions. ORF1 codes for a protein responsible for
kanamycin resistance similar with Tn5 transposone, ORF2 encodes a resistance to ampicillin identical with Tn3 transposone. Plasmid has in A. pasteurianus five copies and in E. coli DH1 about 30 copies per chromosome and it segregation stability in both strains is very high. Based on the data on replication
region, plasmid does not code for a replication protein and origin region is similar with ColE1-like plasmid. |
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| Keywords: | Acetobacter pasteurianus plasmid replicon gene of resistance plasmid stability |
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