Phosphorylation of mCRY2 at Ser557 in the Hypothalamic Suprachiasmatic Nucleus of the Mouse |
| |
Authors: | Nobuhiro Kurabayashi Tsuyoshi Hirota Yuko Harada Mihoko Sakai Yoshitaka Fukada |
| |
Affiliation: | a Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japanb Division of Neuroscience, Children's Hospital, Harvard Medical School, Boston, MA |
| |
Abstract: | Cryptochrome1 and 2 play a critical role in the molecular oscillations of the circadian clocks of central and peripheral tissues in mammals. Mouse Cryptochrome2 (mCRY2) is phosphorylated at Ser557 in the liver, in which the Ser557-phosphorylated form accumulates during the night in parallel with mCRY2 protein. Phosphorylation of mCRY2 at Ser557 allows subsequent phosphorylation at Ser553 by glycogen synthase kinase-3β (GSK-3β), resulting in efficient degradation of mCRY2 by a proteasome pathway. In the present study, we found that mCRY2 is phosphorylated at Ser557 also in the region of the mouse brain containing the suprachiasmatic nucleus (SCN), the central circadian clock tissue. Daily fluctuation of the Ser557-phosphorylation level in the SCN region suggests an important role of sequential phosphorylation of Ser557 and Ser553 in the rhythmic degradation of mCRY2 in both central and peripheral clocks of mice. |
| |
Keywords: | mCRY2 Phosphorylation Glycogen Synthase Kinase-3β (GSK-3β) Suprachiasmatic Nucleus (SCN) Liver Circadian Rhythm |
本文献已被 InformaWorld 等数据库收录! |
|