Immunocytochemical localisation of prolactin and growth hormone in the ovine pituitary |
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Authors: | Dr D M Parry I C McMillen D L Willcox |
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Institution: | (1) Nuffield Department of Obstetrics and Gynaecology and Nuffield Institute for Medical Research, John Radcliffe Hospital, Headington, Oxford, UK;(2) Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Osler Road, OX39DU Oxford, UK |
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Abstract: | Summary Using the peroxidase-antiperoxidase immunocytochemical staining technique, prolactin and growth hormone cells have been identified and described in the ovine pituitary. The image analysing computer, Quantimet 720, was used to assess accurately the size range of the secretory granules in these cell types. The area size distributions of the prolactin and growth hormone granules are similar. An increased proportion of larger granules was observed in the prolactin cells post-partum. Serial sections stained alternately for prolactin or growth hormone confirmed that the cells contain either prolactin or growth hormone but not both. |
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Keywords: | Prolactin Growth hormone Ovine pituitary Immunocytochemistry Granule size |
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