Shotgun collision-induced dissociation of peptides using a time of flight mass analyzer |
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Authors: | Purvine Samuel Eppel Jason-Thomas Yi Eugene C Goodlett David R |
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Affiliation: | The Institute for Systems Biology, Seattle, WA 98105, USA. |
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Abstract: | Parallel collision-induced dissociation (CID) of peptides rather than serial, as is customary, results in loss of the obvious parent-fragment ion lineage available from CID on a single ion. We report proof-of-principle results suggesting the feasibility of parallel peptide CID, referred to here as shotgun CID, for protein identification when using the measured mass accuracies available from a time of flight mass analyzer and currently available search routines such as SEQUEST. Additionally, we report that parent-fragment ion lineage may be reconstructed from information encoded in the chromatographic single ion current traces of peptides. |
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