Neonatally induced tolerance to soluble protein antigens. I. Analysis of B-cell and T helper cell function in mice tolerant to bovine serum albumin or fowl gamma-globulin |
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| Authors: | R B Acres A J Cunningham |
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| Affiliation: | 1. Department of Medical Biophysics, University of Toronto, Canada;2. The Ontario Cancer Institute, 500 Sherbourne Street, Toronto, Ontario M4X 1K9, Canada |
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| Abstract: | High dose tolerance to either bovine serum albumin (BSA) or fowl γ-globulin (FGG) was induced in CBA mice by neonatal injection. Tolerance to BSA lasted about 9 weeks, and that to FGG, about 18 weeks. Splenic B-cell function was analyzed using quantitative in vivo assays and in vitro limiting dilution analysis. Tolerogen-specific IgM- and non-IgM-producing B cells are depleted at least threefold in the spleens of tolerant mice. Tolerogen-specific T-helper-cell function was examined by immunization with haptenated antigens. Analysis of the recovery from tolerance indicates that the return to normal function in the tolerogen-specific B-cell and T helper fractions coincides with the return to normal responsiveness by the whole animal. |
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| Keywords: | To whom all correspondence should be addressed: University College London School of Medicine University Street London WC1E 6JJ England. |
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