Inhibition of urease by Ni ions: Analysis of reaction progress curves |
| |
Authors: | Wies
awa Zaborska Barbara Krajewska Maciej Leszko Zofia Olech |
| |
Institution: | Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland |
| |
Abstract: | The inhibition of jack bean urease by Ni2+ ions was studied in 20 mM HEPES buffer pH 7.0. The inhibition was observed in two systems which differed in the order in which the components of the reaction mixture were mixed. In the first (unincubated), the reaction was initiated by adding urease to the mixture of urea and Ni2+ ions, and in the second (incubated), by adding urea to the mixture of urease incubated with Ni2+ ions prior to the reaction. It was shown that Ni2+ ions are a competitive slow-binding inhibitor of urease. In the first system the inhibition constants are Ki=0.042 mM and Ki*=0.0028 mM, and in the second system Ki*=0.0024 mM. The inhibition was found to involve the rapid formation of a urease-Ni2+complex followed by its relatively slow, reversible isomerization, with forward and reverse rate constants of 0.64 and 0.045 min?1, respectively. |
| |
Keywords: | Urease Inhibition Ni2+ ions Heavy metal ions Enzyme kinetics |
本文献已被 ScienceDirect 等数据库收录! |
|