Duplication of the structural gene for glucosephosphate isomerase and phosphogluconate dehydrogenase inScabiosa columbaria and their phylogenetic implications in the dipsacaceae |
| |
Authors: | R Van Treuren R Bijlsma |
| |
Institution: | (1) Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands |
| |
Abstract: | Zymograms of glucosephosphate isomerase (GPI) and phosphogluconate dehydrogenase (PGD) revealed three isozymes for each enzyme in the plant speciesScabiosa columbaria. Intergenic heterodimers are formed between the polypeptides coded byGpi-1 andGpi-2 and between those coded byPgd-1 andPgd-2, indicating that a GPI and a PGD locus have been duplicated in the past. The ancestral genes assort independently with their duplicated gene, suggesting that the duplications have originated from a process of translocation. Linkage was found only betweenGpi-1 andPgd-2 and betweenGpi-2 andPgd-1, suggesting that the duplicated loci were located on the same translocated chromosomal segment. Both duplications are present in all other examined species ofScabiosa and inCephalaria andKnautia, two other genera of the Dipsacaceae. The generaSuccisa andDipsacus, also belonging to the Dipsacaceae, do not showGpi-1 activity, makingGpi-2 andPgd-1 the most likely ancestral genes. InSuccisa, the isozymes ofGpi-1 andGpi-2 either overlap orGpi-1 has been silenced. The combined results suggest that a chromosomal segment containingGpi-2 andPgd-1 has been translocated before the divergence ofScabiosa, Cephalaria, Knautia, andSuccisa. |
| |
Keywords: | gene duplication glucosephosphate isomerase phosphogluconate dehydrogenase gene linkage Scabiosa columbaria electrophoresis phylogenetics |
本文献已被 SpringerLink 等数据库收录! |
|