High level secretion of recombinant staphylokinase into periplasm of Escherichia coli |
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| Authors: | Sang Jun Lee Il Chul Kim Dong Min Kim Kwang Hee Bae Si Myung Byun |
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| Institution: | (1) Department of Biological Sciences, KAIST (Korea Advanced Institute of Science and Technology), and RCNBMA (Research Center for New Bio-Materials in Agriculture), 373-1, Kusong-dong, Yousong-gu Taejon, Korea |
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| Abstract: | The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm and 5ug/ml to extracellular media, respectively. The rSAK was purified with 59% yield by simple procedures from the periplasm of E. coli. The amino-terminal sequence and human plasminogen activating activity of rSAK were coincided with the authentic SAK. |
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