N-acetylglucosaminyltransferase V mediates cell migration and invasion of mouse mammary tumor cells 4TO7 via RhoA and Rac1 signaling pathway |
| |
Authors: | Yunxue Zhao Jing Li Yanli Xing Jingjian Wang Congxiao Lu Xianliang Xin Meiyu Geng |
| |
Institution: | (1) Department of Molecular Pharmacology and Glycobiology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, P.R. China |
| |
Abstract: | In tumor cells, alterations in cellular glycosylation may play a key role in their metastatic behavior. Using small interfering
RNA against GnT-V, we found that the expression of GnT-V and β1,6GlcNAc branching were significantly reduced which was particularly
accompanied by the arrest in both cell migration and invasion as compared to the negative control. Moreover, the suppressed
GnT-V expression by siRNA technique inactivated the signaling molecules including Rac1, cofilin, Erk and Akt, and activated
RhoA levels in cells lacking GnT-V, but revealed no impact on Cdc42 activity. All these notions disclose for the first time
that GnT-V and β1, 6GlcNAc branching mediate the cell migration and invasion in Rac1-positive and RhoA-negative regulatory
manners.
Yunxue Zhao and Jing Li contributed equally to this work. |
| |
Keywords: | GnT-V β 1 6GlcNAc branching Invasion Migration Rho GTPases |
本文献已被 PubMed SpringerLink 等数据库收录! |
|