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Coexistence of two mitochondrial DNA haplotypes in Japanese populations of Hypera postica (Col., Curculionidae)
Authors:R Kuwata  M Tokuda  D Yamaguchi  J Yukawa
Institution:Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences;and Entomological Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
Abstract:Abstract:  In Japan, the alfalfa weevil, Hypera postica , was first recorded in 1982 from Fukuoka and Okinawa Prefectures and has been spreading to many other prefectures. The weevil seriously infests the Chinese milk vetch, Astragalus sinicus , one of the most important honey resources for honeybees in Japan. Direct sequencing of partial mitochondrial DNA and PCR-RFLP data for alfalfa weevil individuals indicated the coexistence of two haplotypes at various localities in Japan. Molecular phylogenetic analysis for H. postica haplotypes and strains indicated that the two Japanese haplotypes had not derived from a single genetic origin. Based on the results, special comments are made on biological control measures using introduced parasitic waSPS.
Keywords:Astragalus sinicus            apiculture industry  Chinese milk vetch  direct sequencing  PCR-RFLP  the alfalfa weevil
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