Alteration of photosystem II properties with non-photochemical excitation quenching

Oxygen yield from single turnover flashes and multiple turnover pulses was measured in sunflower leaves differently pre-illuminated to induce either 'energy-dependent type' non-photochemical excitation quenching (qE) or reversible, inhibitory type non-photochemical quenching (qI). A zirconium O2 analyser, combined with a flexible gas system, was used for these measurements. Oxygen yield from saturating single turnover flashes was the equivalent of 1.3-2.0 micromole(-) m(-2) in leaves pre-adapted to low light. It did not decrease when qE quenching was induced by a 1 min exposure to saturating light, but it decreased when pre-illumination was extended to 30-60 min. Oxygen evolution from saturating multiple turnover pulses behaved similarly: it did not decrease with the rapidly induced qE but decreased considerably when exposure to saturating light was extended or O2 concentration was decreased to 0.4%. Parallel recording of chlorophyll fluorescence and O2 evolution during multiple turnover pulses, interpreted with the help of a mathematical model of photosystem II (PS II) electron transport, revealed PS II donor and acceptor side resistances. These experiments showed that PS II properties depend on the type of non-photochemical quenching present. The rapidly induced and rapidly reversible qE type (photoprotective) quenching does not induce changes in the number of active PS II or in the PS II maximum turnover rate, thus confirming the antenna mechanism of qE. The more slowly induced but still reversible qE type quenching (photoinactivation) induced a decrease in the number of active PS II and in the maximum PS II turnover rate. Modelling showed that, mainly, the acceptor side resistance of PS II increased in parallel with the reversible qI.