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Protein and cDNA sequences of Bowman-Birk protease inhibitors from the cowpea (Vigna unguiculata Walp.)
Authors:Vaughan A Hilder  Richard F Barker  Reda A Samour  Angharad M R Gatehouse  John A Gatehouse  Donald Boulter
Institution:(1) Department of Biological Sciences, University of Durham, South Road, DH1 3LE Durham, England, UK;(2) I.P.S.R., Trumpington, CB2 2LQ Cambridge, England, UK;(3) Present address: Astromed Ltd., Innovation Centre, Cambridge Science Park, CB4 2LQ Cambridge, England, UK;(4) Present address: Department of Botany, Tanta University, Egypt
Abstract:The protein and gene sequences of the cowpea Bowman-Birk type trypsin inhibitor which confers enhanced insect resistance to transgenic tobacco plants, and of cowpea trypsin/chymotrypsin inhibitors are presented. There are regions of high conservation and high divergence within the 5prime leader, mature protein and 3prime non-coding regions of the Bowman-Birk inhibitors and in the genes which encode them in different members of this family within the Leguminosae. The practical implications of this finding for studies on the evolution of plants and the utilization of these genes for enhancing insect resistance is discussed.
Keywords:cowpea  insect resistance genes  trypsin inhibitors
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