黑线姬鼠两亚种(中国东北地区及俄罗斯远东地区的东北亚种和朝鲜半岛的朝鲜亚种) 的线粒体DNA 序列缺少趋异性

为了检测黑线姬鼠两亚种(来自中国东北地区、俄罗斯远东地区的东北亚种和朝鲜半岛的朝鲜亚种)线
粒体DNA 的变异水平并确定朝鲜亚种的分类地位,我们测序分析了两亚种的线粒体DNA 细胞色素b 的部分序列
(1 054 bp)和控制区的部分序列(860 bp),并与基因库中黑线姬鼠相应的单倍型序列进行了比较。可以看出东
北亚种的序列显示出某些分异,可以被分为2 或3 个亚群,所以我们提出需要更多标本的DNA 分析来确定东北
亚种的分类地位。另外,来自韩国的朝鲜亚种的序列,与来自中国东北地区龙江和哈尔滨的东北亚种的两个亚
群相似(1 个亚群是细胞色素b 的两个单倍型,另1 个是控制区的两个单倍型),表明基于线粒体DNA 序列的遗
传多样性与现今基于形态特征对这些姬鼠的分类所得结果是不一致的。因此我们认为来自韩国的朝鲜亚种是一
个只在形态特异上不同于东北亚种的地方亚种,我们建议通过其他DNA 标记来进一步验证其亚种地位。我们还
认为朝鲜半岛不是最近的冰川期黑线姬鼠残遗种的保护区。

Lack of mitochondrial DNA sequence divergence in two subspecies of the striped field mouse(Apodemus agrarius mantchuricus from Northeastern China and nearby Far-eastern Russia and A.a.coreae from the Korean Peninsula)

To determine the degree of mitochondrial DNA(mtDNA) divergence between two subspecies of Apodemus agrarius(A.a.mantchuricus from Northeastern China and nearby far-eastern Russia and A.a.coreae from the Korean peninsula) and to confirm the taxonomic status of the Korean subspecies,we obtained partial sequences of the mtDNA cytochrome b gene(1 054 bp) and the control region(860 bp) of these two subspecies.These sequences were compared to the corresponding haplotypes of these two subspecies,obtained from GenBank.It was observed that the A.a.mantchuricus sequences were somewhat divergent and could be separated into two or three subgroups,and we propose further DNA analyses with additional specimens to confirm the subspecific status of A.a.mantchuricus. Additionally,the sequences of A.a.coreae,from Korea,were similar to the two subgroups in A.a.mantchuricus(one subgroup of two haplotypes in the cytochrome b; another subgroup of two haplotypes in the control region),from Longjiang and Harbin in Northeastern China,indicating that the genetic diversity of these mice based on mtDNA sequencing is not concordant with their current classification based on morphological characters.We conclude that A.a.coreae from Korea is an endemic subspecies,with only morphological distinctness from A.a.mantchuricus,though we propose further analyses with other DNA markers to reexamine the subspecies status of A.a.coreae. We also conclude that the Korean peninsula did not act as a refugium for A.agrarius during the last glaciation.