Interaction between embryogenic cultures of Scots pine and ectomycorrhizal fungi |
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Authors: | K Niemi Jana Krajnakova Hely Häggman |
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Institution: | (1) Department of Ecology and Environmental Science, University of Kuopio BOX 1627, FIN-70211 Kuopio, Finland, e-mail: karoliina.niemi@uku.fi, Fax:+358-17-163-230, FI;(2) Department of Forest Genetics and Tree Breeding, Forest Research Institute, T.G. Masaryka 22, SK-96022 Zvolen, Slovak Republic,;(3) Finnish Forest Research Institute, Punkaharju Research Station, FIN-58450 Punkaharju, Finland, FI |
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Abstract: | Embryogenic cell masses of three Scots pine (Pinus sylvestris) cell lines K779, K884 and K1009 were cultivated with the ectomycorrhizal (ECM) fungi Laccaria bicolor, L. proxima,
Pisolithus tinctorius, Paxillus involutus and two strains of Suillus variegatus. The average growth ratio of the slowly proliferating cell line K1009 was improved by L. proxima and S. variegatus strain H, while of the rapidly proliferating lines K779 and K884 the non-mycorrhizal controls grew best. The fungi caused
two distinct reactions in embryogenic cultures. In the positive reaction, the shape and light yellow colour of the cultures
resembled the controls, while in the negative reaction the embryogenic cells became brown and necrotic and the fungi grew
aggressively over them. These reactions to the fungi did not correlate completely with effects on the growth ratio. All the
cell lines enhanced the radial growth of S. variegatus H and of P. tinctorius, while the Laccaria species and S. variegatus strain 1 thrived better alone. This study shows that early-stage embryogenic cells of Scots pine and ECM fungi are able to
interact. As some fungi produced a positive reaction or even increased proliferation, they could be used to enhance somatic
embryogenesis of Scots pine. Specific fungi might be used to induce the growth of slowly proliferating cell lines, and knowledge
of positive cell line-fungus interactions could be useful in work with later stages of somatic embryogenesis, such as rooting.
Accepted: 16 July 1998 |
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Keywords: | Embryogenic culture Ectomycorrhizal fungi Proliferation |
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