| 反义c-myc重组腺病毒载体的构建及其对大鼠胸腺淋巴细胞的增殖抑制作用 |
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| 作者姓名： | Liu YG Qi HW Li HZ Su MQ Yu WB Ma YY |
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| 作者单位： | 1. 第四军医大学西京医院,呼吸内科,陕西,西安,710033
2. 第四军医大学西京医院,检验科,陕西,西安,710033 |
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| 摘 要： | 目的:观察反义c-myc重组腺病毒载体对大鼠胸腺淋巴细胞的增殖抑制作用.方法:构建大鼠反义及正义c-myc细菌质粒,并将所得细菌质粒与E1缺失腺病毒质粒导入293细胞系,经共转染得到正义及反义重组腺病毒载体.MTS法检测重组腺病毒载体对大鼠淋巴细胞增殖的抑制作用,RT-PCR检测重组腺病毒载体对c-myc mR-NA表达的影响.结果:反义c-myc重组腺病毒载体可抑制大鼠淋巴细胞增殖,降低淋巴细胞c-myc mRNA的表达.结论:反义c-myc重组腺病毒载体可抑制大鼠淋巴细胞增殖.
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| 关 键 词： | myc基因 反义核酸 腺病毒载体 淋巴细胞 |
| 文章编号： | 1000-6834(2005)02-0220-04 |
| 修稿时间： | 2003年8月26日 |
Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effect on rat lymphocytes |
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| Liu YG,Qi HW,Li HZ,Su MQ,Yu WB,Ma YY.Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effect on rat lymphocytes[J].Chinese Journal of Applied Physiology,2005,21(2):220-223. |
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| Authors: | Liu Ying-ge Qi Hao-wen Li Huan-zhang Su Ming-quan Yu Wen-bin Ma Yue-yun |
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| Institution: | Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi' an 710033, China. |
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| Abstract: | Aim: To observe the antiproliferative effect of antisense recombinant adenoviral vector for c-myc on rat thymus lymphocytes. Methods: Antisense and sense bacterial plasmids for c-myc were constructed. Bacterial plasmids and E1 detected adrenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTS. The expression of c-myc mRNA was detected by RT-PCR. Results: The results showed that antisense recombinant adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation. The expression of c-myc mRNA was decreased after antisense recombinant adenoviral vector for c-myc was transfected into cells. Conclusion: Recombinant antisense adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation. |
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| Keywords: | gene c-myc antisense nucleotide recombinant adenoviral vector lymphocytes |
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