里氏木霉GXC木聚糖酶的研究 |
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引用本文: | 孙建义,许梓荣,顾赛红.里氏木霉GXC木聚糖酶的研究[J].菌物学报,2001,20(2):184-190. |
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作者姓名: | 孙建义 许梓荣 顾赛红 |
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作者单位: | 浙江大学动物分子营养学教育部重点实验室! 杭州 310029,浙江大学动物分子营养学教育部重点实验室! 杭州 310029,浙江大学动物分子营养学教育部重点实验室! 杭州 310029,浙江大学动物分子营养学教育部重点实验室! 杭州 310029 |
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基金项目: | 国家自然科学基金资助项目!(No. 30000118) |
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摘 要: | 研究了里氏木霉GXC产木聚糖酶的条件和酶学性质。结果表明,适宜产酶碳源为乳糖、甘露糖、棉子糖、木聚糖和麸皮,氮源为牛肉膏和酵母膏;产酶的最适初始pH为4.0,30℃培养60h。对以麸皮为碳源的培养液进行纯化的酶特性研究表明,木聚糖酶的最适反应温度为50℃,pH为5.5,该酶在pH5.0(7.0和40℃以下相对稳定。Fe3+和Mn2+对木聚糖酶有较大的促进作用,Cu~2+、Fe~2+和Ca~2+ 具有抑制作用。
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关 键 词: | 里氏木霉 木聚糖酶 产酶条件 特性 |
文章编号: | 1007-3515(2001)02-0184-0190 |
修稿时间: | 2000年11月20 |
STUDIES ON XYLANASE FROM STRAIN GXC OF TRICHODERMA REESEI |
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Authors: | SUN Jian-Yi LI Wei-Feng XU Zi-Rong GU Sai-Hong |
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Abstract: | The condition of xylanase production of Trichoderma reesei GXCand enzyme properties were studied. The suitable carbon and nitrogen sources for xylanase production were lactose, mannose, raffinose, xylan, wheat bran , beef extract and yeast extraction. The optimal condition for enzyme production was initial pH 4.0, shaking at 30℃ for 60 h. Xylanase was purified from the culture supernatant in the medium with wheat bran as carbon source.The optimal temperature and pH for purified xylanase reaction were 50℃ and 5.5, respectively. Xylanase was stable at pH 5.0~7.0 and below 40℃. The xylanase activity was significantly stimulated by Fe3+ and Mn2+, and inhibited by Cu2+, Fe2+ and Ca2+. |
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Keywords: | Trichoderma reesei xylanase condition of enzyme production enzyme properties |
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