Effect of mobile phase additives on resolution of some nucleic compounds in high performance liquid chromatography |
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Authors: | Chun Hua Jin Yoon Mo Koo Dae-Ki Choi Kyung Ho Row |
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Institution: | (1) Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, 402-751 Incheon, Korea;(2) Clean and Technology Research Center, Korea Institute of Science and Technology, 136-791 Seoul, Korea |
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Abstract: | Here we investigate the chromatographic behavior, with reversed-phase high performance liquid chromatography (RP-HPLC) of
nucleic compounds (nucleobases, nucleosides, and nucleotides) on a C18 column in several different mobile phase additives, including1-butyl-3-methylimidazolium tetrafuloroborate (BMIm]BF4]), 1-ethyl-3-methylimidazolium methylsulfate (EMIm]MS]) ionic liquids, ammonium formate, and potassium phosphate. The effect
of the alkyl group length, the imidazolium ring, and the ionic liquid's counterions on retention and resolution of the samples
were tested. The results show the potential application of a used buffer system, ion pairing system, and ionic liquid as mobile
phase additives in liquid chromatography resolution of nucleic compounds. |
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Keywords: | ionic liquid buffer ion pairing RP-HPLC nucleic compounds |
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