Characterization of Mycorrhizas Formed by Glomus sp. on Roots of Hypernodulating Mutants of Lotus japonicus |
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Authors: | M Zakaria Solaiman Keishi Senoo Masayoshi Kawaguchi Haruko Imaizumi-Anraku Shoichiro Akao Akiyoshi Tanaka Hitoshi Obata |
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Affiliation: | (1) Faculty of Bioresources, Mie University, Tsu, Mie, 514–8507 Japan, JP;(2) Graduate School of Arts and Science, The University of Tokyo, Komaba, Meguro-ku, Tokyo, 153–8902 Japan, JP;(3) National Institute of Agrobiological Resources, Kannondai, Tsukuba, Ibaraki, 305–8602 Japan, JP |
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Abstract: | Lotus japonicus hypernodulating mutants, Ljsym78-1 and Ljsym78-2, by the arbuscular mycorrhizal fungus Glomus sp. was characterized. The mutants are defective in systemic autoregulation of nodulation and nitrate inhibition, and form an excess of nodules and lateral roots. The percent root length colonized by the arbuscular mycorrhizal fungi was significantly higher for the mutant than wild-type roots. Detailed assessment of the colonization indicated that the percentage of colonization by arbuscules was increased, but that by external hyphae, internal hyphae and vesicles was decreased, in the mutant roots compared with the wild-type. The succinate dehydrogenase activity of arbuscules, external hyphae and internal hyphae showed similar trends. In addition, the majority of individual arbuscules that formed on the mutant roots had a well-developed and seemingly tough morphology. The results suggest that mutation at the Ljsym78 locus positively stimulates the growth and activity of arbuscules, but leads to reduced growth and activity of hyphae. We report the first identification of Lotus japonicus mutants that show significantly increased arbuscule formation and termed these mutants Arb++. Received 8 August 2000/ Accepted in revised form 19 October 2000 |
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Keywords: | : Arbuscular mycorrhiza Hypernodulating mutants Lotus japonicus Mycorrhizal mutants |
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