| 野猪、家猪及野家杂种猪Leptin基因2、3外显子的SNPs分析 |
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| 引用本文： | 李景芬,于浩,刘娣. 野猪、家猪及野家杂种猪Leptin基因2、3外显子的SNPs分析[J]. 遗传, 2006, 28(4): 413-416 |
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| 作者姓名： | 李景芬 于浩 刘娣 |
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| 作者单位： | 1.东北农业大学动物科学技术学院，哈尔滨 150030；2.黑龙江省农业科学院，哈尔滨150086 |
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| 摘 要： | Leptin是一种内分泌激素（167个氨基酸），主要在脂肪组织中表达，通过下丘脑对机体内能量的消耗和摄取起着重要的调节作用[1]。鉴于此本文对野猪、家猪及野家杂种猪Leptin的2、3外显子进行了SNPs分析，并检测到了多态性。对纯合子片段进行克隆和测序，结果表明在编码区的214位碱基由T突变为C，编码的氨基酸没有发生改变；364位碱基由C突变为G，365位碱基由G突变为C，编码的Arg转变为Ala；426位由G突变为A，编码的氨基酸没有发生改变；451位插入碱基T，造成移码突变；462位由G突变为T。
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| 关 键 词： | 单核苷酸多态性 Leptin 猪品种 |
| 文章编号： | 0253-9772（2006）04-0413-04 |
| 收稿时间： | 2005-05-23 |
| 修稿时间： | 2005-08-31 |
Analysis of SNPs in Exons 2 and 3 of the Leptin Gene in Wild Boar, Swine and Hybrid Swine |
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| LI Jing-Fen,YU Hao,LIU Di. Analysis of SNPs in Exons 2 and 3 of the Leptin Gene in Wild Boar, Swine and Hybrid Swine[J]. Hereditas, 2006, 28(4): 413-416 |
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| Authors: | LI Jing-Fen YU Hao LIU Di |
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| Affiliation: | 1.Animal Science Technical College，Northeast Agricultural University, Harbin 150030, China；2.Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| Abstract: | Leptin is a 167-amino acid hormone mainly expressed in the fat tissue.It serves as an important regulator of energy uptake and consumption through action on the hypothalamus.Herein single nucleotide polymorphisms(SNPs) were detected in exons 2 and 3 of the leptin gene in wild boar,swine and half-bred swine by PCR-SSCP.Cloning and sequencing of homozygous fragments revealed the following variations in the coding region: a silent T to C transversion at nucleotide 214;CG to GC at nucleotides 364 and 365,which converts Arg to Ala;a silent G to A transversion at nucleotide 426;a T insertion at nucleotide 451 resulting in frameshift;and a G to T transversion at nucleotide 462. |
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| Keywords: | Leptin |
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